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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample
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Studies prove that GHK-Cu greatly increases the expression of MMP1 and MMP2 genes and boosts TIMP1 expression at all tested levels
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